Reproductive Medicine

Infertility is defined as the inability of a couple to conceive and have a child after at least one year of regular unprotected intercourse.

Investigating a couple who has a fertility problem requires methodical screening because of the variety of conditions that can lead to infertility.

fertility control – woman

HORMONAL CHECK

  • FSH increases with age as well as in women with few eggs in the ovaries
  • LH may be elevated in Polycystic Ovary Syndrome
  • Estradiol
  • Prolactin: may be increased in cycle disorders
  • Progesterone: for ovulation control on the 21st day of a 28 day cycle
  • Thyroid function control and anti-thyroid antibodies
  • AMI (Anti-Millillary Hormone): Indicates the number of ovaries in the ovaries and predicts the response of the ovaries to stimulating hormones in cases of assisted reproduction.

OVULATION CHECK

Ovulation checck has a central role in the investigation of women as it relates to almost 1/3 of all infertility problems. Some women have some symptoms with their ovulation (slight abdominal pain that lasts for a few hours or brown fluids in the vagina). However, the majority of women do not have such symptoms. Alternatively, a woman may use an ovulation kit that checks for certain hormones in the urine or notes daily changes in body temperature and cervical mucus. However, because these methods are not considered to be very accurate, the doctor can confirm the ovulation by ultrasound examination 2-3 times during the cycle. In addition, in order to confirm ovulation and to determine the proper functioning of the lump, progesterone testing is recommended at that particular day of the cycle indicated by the physician.

Maternal Anatomy

The uterine environment plays a very important role in the fertilization process and its thorough control is necessary before drawing any conclusions about the possible causes of infertility. Possible problems we may encounter during this audit are:

Congenital uterine abnormalities (eg uterine diaphragm, biceps uterus)

Acquired uterine abnormalities (eg endometrial adhesions)

Polyps, fibroids, adenomyosis

All of the above may be related to difficulties in achieving pregnancy or miscarriages. We use one or more of the following tests to diagnose them:

Gynecological ultrasound: intravaginal gynecological ultrasound of the genitals offers an immediate and painless imaging of the uterus. In recent years we have been using a more sensitive form of ultrasound, 3D (3D) gynecological ultrasound that allows us to accurately diagnose congenital uterine abnormalities and has virtually replaced – for this purpose – magnetic resonance imaging.

Hysteroscopy: is a simple examination in which a camera is inserted into the intrauterine cavity through the cervix while the woman is drunk. Using a suitable light source, we can monitor the inside of the matrix on a single screen.

Hydro-ultrasound: a newer imaging method that combines classic gynecological ultrasound (or even 3D ultrasound) with the use of water (for saline accuracy) in the womb. During this examination, saline helps to see clearly the inside of the uterus and diagnose whether or not polyps are present. This way, we can avoid potentially useless hysteroscopy.

PATENCY OF THE FALLOPIAN TUBES

The proper functioning of the fallopian tubes is a prerequisite for achieving pregnancy. The readability of the trumpets is checked by the following methods:

Trumpet: the oldest method used for decades. It must be done in a radiology lab. It consists of infusion through the cervical shaft and X-ray imaging of the endometrial cavity as well as the fallopian tubes.

Ultrasound-tubing (HyCoSy): is the newest method of trumpet control and combines efficacy with simplicity and safety. It consists of injecting a special shade substance into the cavity of the uterus and the fallopian tubes and using ultrasound to diagnose the fallopian tubes. That is, it works the same way as classical tubal ligation, but without the problem of ovarian radiation. It is also much less painful than classical tubal ligation and takes place in the familiar setting of a gynecological practice rather than in a radiology laboratory. In addition, this method allows us to perform a complete examination of the uterus and ovaries in real time.

CONTROL FOR INFECTIONS

  • Gonorrhea
  • Hemophilic vagina
  • HIV
  • Hepatitis B
  • Hepatitis C
  • Cytomegalovirus
  • Scarlet
  • Toxoplasma

FERTILITY CONTROL – MAN

Semen Analysis

The male factor accounts for 40% of the problem in infertile couples.

The evaluation of the male factor is done with the sperm chart. If this is abnormal, it may need to be repeated after 1 month. It is important to get a complete history that includes questions about the spouse’s illnesses, surgeries or injuries, medication use, history of sexually transmitted diseases, occupation, smoking. A specialist urologist may also need evaluation.

In cases of abnormal spermatozoa, the solution may be IVF, either by the classic IVF method, or by the microcytoplasmic injection of a sperm – ICSI, or a testicular biopsy. With these techniques, many couples with male fertility problems have had their own children.

Instructions for semen collection

  • The method of obtaining semen should be in accordance with the following instructions:
  • There should be no intercourse or ejaculation for 2 days. This period is calculated without counting the day of contact and the day of the examination.
  • The collection is usually done in the laboratory.
  • Sperm can also be collected in your home as long as the transfer does not take more than an hour. When transporting keep the sample at 18 – 30 ° C, away from the sun.
  • If the collection is made at home, inform the laboratory of the exact time of collection.
  • Before taking, wash your genitals and hands thoroughly with soap and rinse with plenty of water. Wear clean underwear.
  • The collection is done in a sterile container that can be purchased from a pharmacy.
  • Collection should be done by masturbation and without the use of a condom.
  • Try to gather all the material in the sterile container so that the test is reliable. If this is not the case, please inform the laboratory.

Sperm – Normal values

  • Volume: 2.0ml or greater
  • pH: 7.2-8.0
  • Sperm count: 15,000,000 / ml or greater
  • Total number of sperm: 40,000,000 at least ejaculate
  • Mobility: 40% with excellent overall and moderate movement
  • Morphology: Minimum 3% of normal forms
  • Leukocyte count: Less than 1,000,000 / ml

(DNA FRAGMENTATION)

DNA fragmentation is the cleavage in one or both strands of sperm chromosome DNA. When this happens we may have unexplained conception (with normal sperm count) or miscarriages.

Normal DNA fragmentation values ​​are up to 25%. Factors responsible for increased DNA fragmentation include infections, smoking, exposure to environmental pollutants, advanced age, varicocele, recent episodes of high fever, and conditions predisposing to elevated testicular temperature (eg, laptops between legs) .

To improve DNA fragmentation values, we recommend a diet rich in fruits and vegetables, taking antioxidant supplements, vitamins C, E, coenzyme Q 10, carnitine, selenium, zinc, folic acid, smoking cessation, cure for infections. Screening for sperm DNA fragmentation is done just like sperm count (with simple sperm production by the man).

Cytogenetic Semen Examination (FISH TECHNICAL)

International literature reports that infertile men with normal peripheral blood karyotype have a higher incidence of chromosomal abnormalities in sperm as compared to men with normal sperm counts. Indicatively, the most common chromosomal abnormalities are abnormal numbers of racial chromosomes or structural chromosomal abnormalities (eg displacement of genetic material from one chromosome to another). As a result, an increased number of embryos with chromosomal abnormalities can be created, thereby reducing the chances of successful IVF attempts.

Criteria for sperm cytogenetic examination and sperm apoptosis include:

Recurrent biochemical / reciprocal pregnancies in women under 35, with normal peripheral blood karyotype

Poor quality embryos in women under 35 with normal peripheral blood karyotype

Inability of the sperm to fertilize

Men who have undergone radiotherapy or chemotherapy

Men with oligo-patient-teratozoospermia

Unexplained infertility

TREATMENTS

CYCLE MONITORING

Monitoring the menstrual cycle is the simplest form of assisted reproduction. The help offered is in monitoring the development of the ovaries, in the period of ovulation and in the planning of sexual intercourse. We do not stimulate the ovaries with medication and so this is the most natural way of assisted reproduction

Procedure: Normally every woman produces and releases one egg every month. If it has a 28-30 day cycle, ovulation (ie the release of the egg from the ovary) occurs between the 12th and 16th day of the cycle. In the menstrual cycle tracking method, an ultrasound is performed on the 10th day of the cycle (day 1 is the first day of the period). This ultrasound will show us the size of the dominant ovary and the thickness and nature of the endometrium.

When the diameter of the ovary reaches 16 mm, the woman is advised to start using the ovulation tests (each day), which can be obtained from the pharmacy. When the ovulation test is positive, the couple is advised to begin sexual intercourse for the next three days, starting on the day of the positive ovulation test.

Which women should be given this type of treatment?

  • Women who have a normal menstrual cycle (28-30 days) and ovulate every month
  • Women with unexplained short-term infertility (less than 6 months)
  • Women without but hormonal or anatomical problems

Pros

  • It is the simplest form of assisted reproduction
  • The woman does not take any medicines
  • There is no chance of multiple pregnancy (since we do not stimulate the ovaries)
  • The cost of treatment is very low

Cons

  • Pregnancy rate is rather low (less than 10%)
  • The couple has to be patient, as it may take longer to conceive with this method.


OVULATION INDUCTION

This is a procedure to enhance ovulation in order to increase the number of eggs released per cycle and maximize the chances of conception. In cases of unstable menstrual cycle or known aneurysms we use formulations to induce ovulation such as clomiphene citrate and letrozole (FEMARA).

Indications

Women with aneurysms (regular transvaginal ultrasound)

Mild ovarian stimulation in women with low response to classic stimulation (poor responders)

Age under 35 years

In cases where classic ovarian stimulation is not permitted for medical reasons

In women with a history of breast cancer (Letrozole / Femara)

The procedure is monitored by regular intermittent ultrasound and blood hormone screening to evaluate the effect of the hormone preparation and to tailor the dosage according to the findings of the tests.

Clomiphene Citrate: Clomiphene citrate is the substance that binds to estrogen receptors in the brain, resulting in a false message to the brain that there is a lack of estrogen. This message stimulates the brain to produce via the pituitary glands greater amounts of endogenous gonadotrophins (FSH, LH) than those produced in a natural cycle, which in turn stimulate the ovaries to produce more than one follicle.

It is usually administered from the 3rd to the 7th day of the cycle at a dosage of 100mg daily, with the dose being increased to 150mg if the ovarian response is not satisfactory. The follicles, which are larger than 18mm in gross diameter, are considered ripe, in which case the chorionic gonadotropin hormone hCG is administered to cause the final follicles to mature, which will, in 32-36 hours, lead to the ovarian follicle. If pregnancy is not achieved after 4-6 cycles of treatment then more active preparations such as gonadotrophins are used.

Benefits of treatment are the low cost and ease of recruitment, while the disadvantages include low pregnancy rates of 8-15% per cycle, the possibility of twin or ectopic pregnancy, the possibility of ovarian hyperstimulation, drug failure and all side effects vasomotor disturbances and alteration of cervical mucus fluid.

Letrozole (FEMARA): It is a substance that selectively inhibits the aromatase enzyme and drastically reduces tissue production of estrogen. The main indication is its supplementation in the case of women who have undergone breast cancer surgery to protect them from the hormones produced by the body.

Their ability to inhibit estrogen production is also used in infertility to induce ovulation. Low estrogen levels cause the pituitary gland to produce greater amounts of endogenous gonadotrophins (FSH, LH) than those produced by natural cyclone, in turn they stimulate the ovaries to produce more than one follicle.

It is usually administered from the 3rd to the 7th day of the cycle at a dosage of 4mg daily, with the dose being increased to 6mg if the ovarian response is not satisfactory. The follicles, which are larger than 18 mm in gross diameter, are considered ripe, whereby the hormone chorionic gonadotropin hCG is administered in order to cause the final follicular maturation which, after 32-36 hours, will lead to the ovarian follicle.

Benefits of treatment are low cost, ease of recruitment and safety in use in women with breast cancer. The disadvantages include a low pregnancy rate of 8-15% per cycle, the possibility of twin or ectopic pregnancy, the possibility of ovarian hyperstimulation, drug failure and other side effects with the main headache and overdose.

Unlike clomiphene citrate, letrozole does not cause cervical mucus deterioration, so its use need not be accompanied by intrauterine insemination.

Intrauterine Insemination (IUI)

IUI is the direct injection of sperm into the internal cavity of the uterus (endometrium) and aims to increase the number of sperm inserted into the fallopian tubes, thereby aiding egg fertilization. Regardless of whether a sample of semen is produced fresh or defrosted, it must be processed to separate the vital (active) spermatozoa from the remaining constituents of the spermatic fluid. If the semen is not properly processed and placed as it is in the uterus, substances known as prostaglandins may cause uterine contraction, which eliminates infusion while at the same time increasing the risk of infection.

The procedure of insemination is relatively responsible and does not cause pain or discomfort to the patient. The specialist first inserts the vaginal dilator in order to identify the best access to the cervical orifice. After a vaginal wash is performed, a thin, flexible catheter is inserted through the cervix into the uterus. Its exact location is determined using abdominal ultrasound. The sperm are slowly removed in the center of the uterine cavity while the ultrasound confirms the successful infusion.

One obvious disadvantage of insemination when it comes after hormonal ovarian stimulation is the possibility of multiple pregnancies and the associated risks that they may follow, such as ovarian hyperstimulation, premature birth and increased rates of miscarriage.

Success rates vary with age, the couple’s fertility problem, and whether the treatment involves ovarian stimulation (increased number of eggs) or is applied during a physical cycle (one egg only). On average the success rate with the insemination method is 20% for each new attempt.

 

IVF IN NATURAL CYCLE

The first successful IVF attempt in Manchester in 1978 was by the natural cycle method. Although over the years the use of ovarian stimulation medications has changed a lot, in recent years the natural cycle method has become increasingly used as an alternative to medication use.

The natural cycle is the method in which we do not administer drugs to stimulate the ovaries and obtain many eggs, as is done in classical IVF. On the contrary, we are watching the ovary which the woman normally produces herself during the cycle.

Monitoring is done by vaginal ultrasound as well as hormone measurement in the blood. When the follicle is large enough, at about 16-17 mm, then a gonadotropin injection is made for its final maturation (Pregnyl, Ovitrelle) and, after 36-38 hours, the egg is taken. Once the egg is harvested and fertilized, the resulting embryo is placed in the uterus using the classic method of embryo transfer, 2-3 days after egg collection.

The advantage of the natural cycle is that we do not administer any medication to stimulate the ovaries. Although in-person medications have been used for 25 years and do not appear to be responsible for health effects, many women appear reluctant to take injectable hormones. By the natural cycle method, the stage of stimulation is bypassed, and the only drugs used are non-injectable post-ovulation drugs, which aim to improve the chances of embryo implantation in the uterus and achieve pregnancy.

In addition, the main complications of classical IVF are avoided: Ovarian Overstimulation Syndrome and Multiple Pregnancy.

This method is used in women who either cannot take medicines for various medical reasons or have poor response (Poor responders). It can also be used in women who have made multiple failed attempts with poor quality embryos.

The disadvantage of the physical cycle is that the success rates of the method are significantly lower than that of classical IVF using drugs. The chance of finding a good egg during ovulation, fertilizing and developing a good quality embryo in the laboratory is about 50-60%. Once a good quality embryo is transferred, the chance of pregnancy can be as high as 35%, depending on the age of the woman.

In summary, the natural cycle method can be used with quite good success rates either in women who do not wish to take medications to stimulate their ovaries or in women whose response to medication is poor.

IVF (MEDICINE USE)

For many couples with fertility problems, in vitro fertilization (IVF) is the best solution for achieving pregnancy. It can be used to treat a variety of causes of infertility, such as mild sperm problems, endometriosis, fallopian tubes, or unexplained infertility. In the process of classical IVF, the eggs are fertilized by the sperm in the laboratory environment (instead of the female tubes). Fertilization can be achieved either by the classical IVF procedure or by the method of Intra-cytoplasmic sperm injection (ICSI).

The story of IVF

Robert G. Edwards was set to become the most famous biologist of his time when he announced, on July 25, 1978, the birth of Louise Brown, the first “tube child” in England, together with the obstetric surgeon Patrick Steptoe and midwife Jane Purdie. Since then, developments have been rapid: there is a breakthrough when the announcement of the successful microbrewing with intracellular sperm injection (ICSI) in 1992 (Palermo) and its variants, MESA (Scherb and Asch & 1992) , 1993). Male infertility is thought to have been virtually eliminated. In Greece, the first child by IVF was born on January 20, 1982 and was the 13th child in the world.

The stages of IVF – Procedure

The IVF cycle includes the following stages:

Investigation and diagnosis of infertility

Preliminary examinations

Ovarian stimulation with medication (12-14 days), with the aim of developing many follicles. We select the type of protocol based on the specifics of each woman’s cycle, age, previous efforts and other factors that we evaluate

Monitoring with a series of ultrasound and hormonal determinations. Monitoring is done every two to three days to optimize your daily dosage of medication and to optimize your effort.

Induction of final maturation of ova (single evening injection). On the day that the follicles have matured sufficiently, the last injection is given, which will schedule the final maturation of the eggs and follicular function.

Ovulation (Egg collection). Eggs are taken intermittently by puncture and continuous ultrasound guidance. The ovulation is short in duration (10-30 minutes) and practically painless because it is performed under drunkenness.

Seeding (Collection of sperm). In cases of azoospermia, testicular biopsy (TESE) is suggested

Egg fertilization by the sperm. At this laboratory stage, the eggs come in contact with the sperm. When the semen has normal parameters, the classical IVF is chosen as the method of fertilization. There is no intervention in the classical method. For many couples with poor sperm quality, where the sperm is unable to fertilize the egg itself, ICSI-Intracytoplasmic Sperm Injection is the method of treatment that allows successful egg fertilization even in severe cases. The next day, the embryologist checks and records the percentage of normal fertilized eggs

Embryo culture After fertilization the embryos are cultured in the laboratory for 2 – 6 days, until the blastocyst stage. Proper cell division is a key criterion for their quality

Embryo transfer Select the best embryos and transfer them to the uterine cavity. Two to six days after ovulation the embryos are transferred to the uterus. It is a painless process that does not require drunkenness. With the help of a thin catheter, the embryos are transferred to the endometrial cavity

Pregnancy check (13 days after embryo transfer with blood sampling to determine gonadotropin beta-hormone)

Interventional ultrasound clinical pregnancy screening (4 weeks after embryo transfer).

Control of progressive pregnancy at 12 weeks gestation ie 10 weeks after embryo transfer.

Optional procedures (depending on background)

Freezing of embryos or ova. Freezing allows the eggs, sperm and embryos to be maintained for an extended period of time (for a period of five years or more). By applying the new method of vitrification, vitrification, it seems that survival and pregnancy rates are higher than progressive controlled freezing.

Assisted Hatching is a laboratory technique that facilitates embryo hatching. Recommended when the fetal translucent zone is thick or hard, in cryopreserved embryos, in women with previous

ENDOMETRIAL PREPARATION AND EMBRYOTRANSFER OF BLASTOCYSTS

For many years, couples undergoing IVF treatment have been able to freeze and cryopreserve non-transferable embryos at the end of treatment. The embryos are stored in liquid nitrogen and can be used at a later date.

Embryo cryopreservation is performed with the new method of vitrification, after embryos reach the blastocyst stage (day 5 or 6), to ensure that only embryos with good growth potential are stored. When decided to use, these embryos are thawed, and at the appropriate stage of the woman’s cycle are transferred to the endometrium.

GETTING SEMINAR WITH TEST BIOLOGY OR EPIDEMIDA

Sperm Surgery (TESE)

Sperm surgery is recommended in case of azoospermia, ejaculation failure or induced ejaculation failure. The procedure is performed with intravenous or local analgesia. The testicular tissue obtained is specially processed by the Embryologist for the purpose of finding sperm. After isolation of spermatozoa, the ova are fertilized by the method of micro-fertilization. Excess sperm may be frozen for future use. There are various methods of sperm surgery, either by the epididymis or the testes.

Obtaining sperm from the epididymis

  • Microsurgical epididymal sperm aspiration (MESA): Recommended in cases of obstructive azoospermia with normal spermatogenesis. It involves surgical excision of the epididymis using a microscope and suction of the epididymal fluid to find spermatozoa.
  • Percutaneous epididymal sperm aspiration (PESA): Recommended in cases of obstructive azoospermia with normal spermatogenesis. It is performed by puncturing the epididymis using a 19-21G needle.

Taking sperm from testicles

  • Fine needle aspiration (FNA): Recommended in cases of obstructive azoospermia with normal spermatogenesis. The testis is punctured using a 21G thin needle.
  • Testicular sperm aspiration (TESA): Recommended in cases of obstructive azoospermia with normal spermatogenesis.
  • Testicular biopsy (testicular sperm extraction – TESE): Recommended in cases of obstructive and non-obstructive azoospermia and involves open biopsy and collection of small pieces of testicular tissue.

Methods of suctioning the epidermis from the epididymis (MESA, PESA) or the testes (TESA) are simpler, but are largely “blind”, causing damage to the testicular tissue, such as hematoma, fibrosis and testicular atrophy. Testicular biopsy (TESE) is suggested by international literature as the most effective method of finding sperm and is associated with a lower rate of complications.

FREEZING – STORAGE OF Semen and Tissue

Historical data

The first mention of the possibility of freezing semen was made in 1776 by Spallanzani after successful experiments with freezing – thawing human semen in the snow. But in fact, the first semen freeze occurred in 1949 when Polge and his colleagues observed the cryoprotectant effect of glycerol on avian semen that was frozen at -76 degrees Celsius. It was followed in 1953 by Sherman and Bunge who first frozen human sperm in a 10% solution of glycerol and dry ice. The first pregnancy from sperm that had been frozen in glycerol solution and liquid nitrogen was achieved by the same research team a few years later. The first human semen cryopreservation banks were created in the 1970s, and the birth of the first infant with Louis Brown in 1979 gave new impetus to the improvement of semen freezing techniques.

How to freeze – freeze sperm?

The semen can be frozen through a suitable procedure and stored in nitrogen-containing containers at -196 degrees Celsius. The most commonly used method involves adding to the sperm a suitable volume of cryoprotectant such as glycerol at a concentration of 5-10% and then freezing the sample with or without the use of a cooling gradient. Although no significant changes in results have been made in recent years to improve the cryopreservation method, more complex solutions containing other non-glycerol substances such as egg yolk, soy lecithin and citric acid have also been used. Frozen semen can be preserved without losing its properties and fertility for many years or even decades.

  • Indications – Advantages of Freezing – Sperm Cryopreservation.
  • In men who are going to undergo chemotherapy or radiation due to cancer, this is expected to significantly affect their fertility. In men who are about to undergo orchectomy or prostatectomy due to benign or malignant tumor in order to maintain their fertility in the future.
  • In men who are about to undergo neutering (vasectomy – ligation of the semen) so that their fertility can be reversible.
  • In males with poor quality of life such as excessive smoking, high and daily alcohol consumption, daily exposure to occupational risk factors (chemicals, radiation, excessive heat) that they wish to maintain through sperm cryopreservation in their distant future.
  • Couples following extracurricular cycles but not being able to physically present their spouse on the day of vaccination (long business trips, unforeseen events at work), etc.
  • In men who have an erection defect and cannot ejaculate or have a complete lack of spermatozoa, sperm can be directly collected from the epididymis and testis and the collected spermatozoa to be frozen for use.
  • In men who have had an accident (spinal cord injury) and cannot often ejaculate, the amount and quality of sperm decreases over time. In these cases, storing – preserving sperm as early as possible can greatly protect their fertility.
  • In men who wish to give birth at a later time and want to secure their reproductive potential, as a foreshadowing move.
  • In men who have been successfully treated for improved sperm quality but are less likely to maintain this effect, they can freeze sufficiently at this time in their quantity and quality of sperm.
  • In men with severe oligoasthenia when multiple ectopic material is required to be concentrated.

The possibility of establishing semen banks and using semen donation at any time for couples facing severe azoospermia problem was provided.

Embryo Freezing

 

In vitro fertilization since its inception in 1978 has been the most important scientific breakthrough in the treatment of infertility. Developments since then have eased the effectiveness of the method to very high levels. An important role in this direction has been the development of a new innovative method of embryo cryopreservation, vitrification. The vitrification method allows for safe preservation of embryos, achieving survival rates exceeding 98% after thawing!

The high efficacy of the new cryopreservation method has enabled experts to adopt new more effective practices in the implementation of IVF.

One such emerging and important practice, which in recent years has been gaining ground more and more internationally, is the planned embryo cryopreservation and transfer to the next cycle.

In some cases during an IVF cycle it may be found that the endometrium does not have the ideal conditions for implantation. Factors that may affect the function of the endometrium and therefore the implantation process may be the medication itself used during IVF, ovarian hyperstimulation syndrome, as well as pathological findings that may be found during treatment such as endometrial polyps, very thin endometrial etc. Today and thanks to the vitrification method it is possible to cryopreserve all the embryos for as long as needed until they are secured. the ideal conditions in the endometrium before embryo transfer of valuable embryos. Scheduled embryo cryopreservation and embryo transfer in the next cycle is a new modern approach that allows us to deliver embryos only when endometrial conditions are ideal, thereby maximizing the likelihood of pregnancy.

The maintenance of the resulting excess embryos, in a cycle of IVF, has been a well-known and recognized method for many years. But thanks to the vitrification method the efficiency of embryo maintenance has been maximized! Nearly 100% of embryos survive after thawing and remain as capable of pregnancy as fresh embryos. This new advanced feature allows the couple to complete their family without the need for repetitive therapeutic procedures and implements the plan: “a Stimulation Therapy – a Family”!

Experience so far has shown that the results of implantation of embryos that have been cryopreserved by vitrification are as good as those obtained from embryo transfer of fresh embryos, and in some cases even better, because of the ideal endometrial conditions that we guarantee! Both redundant embryo cryopreservation and planned embryo cryopreservation and subsequent embryo transfer are powerful tools for the individual treatment of each couple.

OVES CROSSROADS – FERTILITY MAINTENANCE

Cryopreservation of egg-laying eggs is a method that has recently begun to be implemented in the field of assisted reproduction and is applied in cases such as:

  • Women with early ovarian failure
  • Women who are going to undergo oncology treatment and there is an increased risk of losing fertility
  • Women who want to maintain their fertility and postpone the birth of a family
  • Couples wishing to avoid embryo freezing for ethical or religious reasons

The first pregnancy report from frozen eggs was obtained by Chen in 1986. Since then, few children have been born worldwide because the process of freezing eggs by the freezing method did not have high success rates. The main reason was the difficulty in cryopreservation of cells with high water content, such as eggs.

On the contrary, the recently used vitrification method, according to the international literature, seems to be successfully used for cryopreservation of anoxic eggs. The survival rate of eggs after thawing is over 90%, the fertilization rate is about 70% and the pregnancy rate is more than 40%. In order to collect the eggs for cryopreservation, the woman undergoes hormonal ovarian stimulation, as in the case of IVF. The procedure takes approximately 9-12 days during which it is subjected to ultrasound and blood sampling for analysis, at regular intervals. Once the eggs have matured sufficiently, they are removed with a thin needle placed through the vagina under ultrasound guidance. The procedure is not painful, it takes a while and the woman is intoxicated, a form of narcolepsy. The eggs collected are frozen immediately to -196 degrees Celsius and remain stored until the woman thinks it’s time to become a mother. “

Cryopreservation of egg-laying eggs is a promising method that touches on the modern living conditions of Greek women and seems to offer much in the field of assisted reproduction.


Egg Donation

In vitro fertilization using Danish eggs concerns women who:

  • They treat early menopause
  • They have a history of repeated failed IVF attempts
  • History of recurrent miscarriages
  • History of low quality eggs
  • History of hereditary disease

It is a well-documented scientific process with excellent results that in specialized units the success rates are over 50%.

This unit has been working in this unit for over 15 years with excellent results and within the framework of responsibility and application of particularly stringent scientific and ethical criteria.

According to the Greek Legislation in force since 2005, donors are healthy Greek women under the age of 35 who, on their own initiative and voluntarily donating or eggsharing, anonymously consent to the offer of a specific number of eggs.

  • Donors give a detailed individual and family history and are screened for
  • communicable diseases (hepatitis, AIDS, syphilis etc.),
  • haematological diseases (Mediterranean anemia, cystic fibrosis),
  • chromosomal abnormalities with karyotype.

Exterior features are also simulated between the two women.

The recipient of the ova should be screened for the well-being of its uterus and appropriate hormonal preparation of the uterus for embryo reception. The waiting time for finding eggs is between 5-6 months.

GENETICS

PREIMPLANTATION GENETIC DIAGNOSIS (PGD)

Preimplantation genetic diagnosis (PGD)

Pre-implantation genetic diagnosis (PGD) is a laboratory procedure performed prior to embryo transfer to identify hereditary genetic diseases in the fetus. In this way, we are able to detect gene mutations associated with specific diseases before the embryo transfer and implantation stage, thereby preventing pre-pregnancy illnesses and, consequently, giving birth to normal children. Specific gene mutations (PCR) are responsible for the occurrence of known, congenital and hereditary diseases in the fetus. The technique can be applied to either an anesthetized mature egg (1st polar body biopsy), a 3rd day embryo (blastomeride biopsy), or a blastocyst (trophoblast biopsy).

Patients indicated for PGD are:

  • Couples – carriers of monogenic or sexually transmitted disease.
  • Couples wishing to give birth to a tissue-compatible baby who will become a bone marrow donor to another suffering child in the family.

Examples of diseases that are used in pre-implantation genetic diagnosis are β-Mediterranean anemia, cystic fibrosis, etc. With the continued advancement of genetics, it is expected to identify more genes responsible for specific hereditary diseases, allowing more diseases and even cancers to be avoided.

Preimplantation genetic diagnosis is advantageous over conventional methods of prenatal screening, namely amniocentesis and trophoblast biopsy, because it avoids the possibility of pregnancy termination if the test results in positive fetal disease. Finally, it should be made clear that the method seeks out specific diseases and does not exclude the birth of a child with another form of genetic damage.

PREIMPLANTATION GENETIC SCREENING, PGS

PGS (Preimplantation genetic screening)

The purpose of PGS is to identify structural or arithmetic chromosomal abnormalities (aneuploidies), including chromosomal lack of addition or translocation, in embryos of couples known to be fertile. 40 to 60% of pre-implantable embryos are aneuploid, which is a possible reason for the relatively low implantation efficiency in both physical conception and IVF. The main indications for the use of PGS in IVF treatments include woman’s advanced age, recurrent implantation failure, and recurrent miscarriages. The success of PGS depends to a large extent on the technical ability, quality of the embryo and the presence of the mosaic in pre-implantation embryos. Isolated cells are screened for structural or arithmetic abnormalities of a series of chromosomes using cytogenetic methods (fluorescence in situ hybridization, FISH). Today, third-day embryo biopsy is the most common method. However, blastocyst biopsy is quickly becoming the most preferred method due to the reduced likelihood of a mosaic and an increase in the amount of DNA available for diagnosis.

Patients indicated for PGS are:

  • Couples that women are older. More than half of the embryos from women aged 35-40 have chromosomal abnormalities. This percentage increases much as a woman’s age increases.
  • Couples with a history of repeated miscarriages and / or failed IVF attempts.

The benefits of PGS are:

  • Higher chance of pregnancy
  • Reduced risk of miscarriage
  • More confidence in carrying a fetus, avoiding the risks associated with twin or multiple pregnancy
  • Reduced number of IVF cycles required to achieve pregnancy
  • There is a great deal of debate today as to whether PGS is finally a technique that can help and increase pregnancy rates. This is due to the mosaicism presented by the embryos and can lead to a misdiagnosis. For this reason, research is ongoing to establish whether pre-implantation genetic control can be established in the field of assisted reproduction and to effectively assist infertile couples.

Concerning PGS technology, two newer methods that offer significantly increased diagnostic accuracy are comparative genomic hybridization (CGH) and New Generation Sequencing (NGS).

Investigating a couple with fertility problems requires methodical screening due to the variety of conditions that can lead to infertility.